我的试验方案：
Superoxide radicals were generated by pyrogallic acid method (Meng et al., 2005),and the method was innovated slightly. The system contained 4.5 ml of Tris–HCl buffer (0.05 mol, pH 8.2), 1 ml of sample solution, 0.4 ml of pyrogallic acid(3.0 mmol), and 0.5 ml of thick hydrochloric acid for termination the reaction. Thesolution was incubated at 25℃and determined at 299 nm. In blank, sample solution was substituted with Tris–HCl buffer.
The antioxidant activity of the sample was evaluated according to the following
formula: Scavenging rate E% = (1 - A/A0) *100%, where A was the absorbance
of the test sample, and A0 was absorbance of the blank.
但是阳性对照物VC在50ug/ml时的清除率才为52%，与文献上差很多。。另一阳性对照物BHT则随着浓度的增加清除率反而下降，难道BHT不能清除超氧阴离子，反而促进超氧阴离子的生成？
请问大家有遇到这种情况吗？还是这个方案有错。。。如果大家有邻苯三酚自氧化法测定抗氧化活性的其他方案，效果和重复性还不错的话，请大家告诉我。。
我也做过，结果是负清除，所以放弃
大家提提建议！不管好坏。。
我们之前也做出过相似的结果，后来放弃了
我也在做但能力太差无法解释
我也做过结果也是很不好，后来就不用这个了
学习一下
学习以下 我做也出现了这种情况 你尝试改变以下他们的浓度看下
我也做过，结果很奇怪，解释不了，就放弃了
Superoxide radicals were generated by pyrogallic acid method (Meng et al., 2005),and the method was innovated slightly. The system contained 4.5 ml of Tris–HCl buffer (0.05 mol, pH 8.2), 1 ml of sample solution, 0.4 ml of pyrogallic acid(3.0 mmol), and 0.5 ml of thick hydrochloric acid for termination the reaction. Thesolution was incubated at 25℃and determined at 299 nm. In blank, sample solution was substituted with Tris–HCl buffer.
The antioxidant activity of the sample was evaluated according to the following
formula: Scavenging rate E% = (1 - A/A0) *100%, where A was the absorbance
of the test sample, and A0 was absorbance of the blank.
但是阳性对照物VC在50ug/ml时的清除率才为52%，与文献上差很多。。另一阳性对照物BHT则随着浓度的增加清除率反而下降，难道BHT不能清除超氧阴离子，反而促进超氧阴离子的生成？
请问大家有遇到这种情况吗？还是这个方案有错。。。如果大家有邻苯三酚自氧化法测定抗氧化活性的其他方案，效果和重复性还不错的话，请大家告诉我。。
我也做过，结果是负清除，所以放弃
大家提提建议！不管好坏。。
我们之前也做出过相似的结果，后来放弃了
我也在做但能力太差无法解释
我也做过结果也是很不好，后来就不用这个了
学习一下
学习以下 我做也出现了这种情况 你尝试改变以下他们的浓度看下
我也做过，结果很奇怪，解释不了，就放弃了