本人因实验购买了rhGM-CSF 和 rhIL-4 ,规格都是10μg/支,是干粉剂型,老师要求实验这星期要开始,但令我头痛的是这两种试剂实验所需的浓度我不知怎样配制,请求各位大侠帮忙
rhGM-CSF biological activity :the ED50 was determined by the dose-dependent co-stimulation (with human IL-4)of MC/9 cells is <2.0ng/ml. corresponding to a specific activity of >=5× 100000 units/mg
rhIL-4 biological activity :the ED50 was determined by the dose-dependent co-stimulation of the proliferation of human TF-1 is <2.0ng/ml. corresponding to a specific activity of >=5× 1000000 units/mg
我想配成的浓度均为1000U/ml, 请问各位大侠1μg这两种试剂分别有多少个单位,
!!!!
调研一下文献,先要配成储备液。
rhGM-CSF biological activity :the ED50 was determined by the dose-dependent co-stimulation (with human IL-4)of MC/9 cells is <2.0ng/ml. corresponding to a specific activity of >=5× 100000 units/mg
rhIL-4 biological activity :the ED50 was determined by the dose-dependent co-stimulation of the proliferation of human TF-1 is <2.0ng/ml. corresponding to a specific activity of >=5× 1000000 units/mg
我想配成的浓度均为1000U/ml, 请问各位大侠1μg这两种试剂分别有多少个单位,
!!!!
调研一下文献,先要配成储备液。