Separation of Astragalus Saponins by ReversedPhase High Performance Liquid Chromatographyand Evaporative Light Scattering Detection
Key Wards
Column liquid chromatography
Evaporative light scattering detection
Astragalus species
Saponin
Summary
A new HPLC method permitted the separation of 13 triterpene glycosides isolated from different
AstragaJus species within 40 min. A water / acetonitrile gradient was used as eluent and5 Ixm RP-18 material as stationary phase. By using an evaporative light scattering (ELS) detector,the main saponins of A. membranaceus could be detected at levels as low as20.0 I~g" mL 1. This method facilitated distinction of different Astragalus species as well as theanalyses of market products containing A. membranaceus. The results showed variations from0.019 to 0.184% in the total saponin content of the market products.
Introduction
In our target=_blank>原文2.doc (132.0k)
Key Wards
Column liquid chromatography
Evaporative light scattering detection
Astragalus species
Saponin
Summary
A new HPLC method permitted the separation of 13 triterpene glycosides isolated from different
AstragaJus species within 40 min. A water / acetonitrile gradient was used as eluent and5 Ixm RP-18 material as stationary phase. By using an evaporative light scattering (ELS) detector,the main saponins of A. membranaceus could be detected at levels as low as20.0 I~g" mL 1. This method facilitated distinction of different Astragalus species as well as theanalyses of market products containing A. membranaceus. The results showed variations from0.019 to 0.184% in the total saponin content of the market products.
Introduction
In our target=_blank>原文2.doc (132.0k)