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发布者: src
发布时间: 2025-12-28 04:19:19
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发布者: src
发布时间: 2025-12-27 03:18:18
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发布者: 王跃
发布时间: 2025-12-26 16:51:02
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发布者: sm
发布时间: 2025-10-19 20:57:04
啊大大
发布者: fhsrths
发布时间: 2025-10-08 00:47:02
afgdfhgadfh
发布者: 王跃
发布时间: 2025-08-29 15:59:58
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发布者: qer
发布时间: 2024-04-28 16:21:44
1
发布者: src3
发布时间: 2024-04-19 12:47:11
https://yaoxue8.com/e/tool/gbook/?bid=1
发布者: qer
发布时间: 2024-04-18 21:13:46
1
发布者: 朱新录
发布时间: 2022-11-29 12:10:05
生物技术制药第六章
一、单选题
1、下列哪一项不属于固定化酶（细胞）的评价指标（C）
A.固定化酶（细胞）的活力
B.偶联率及相对活力
C.固定化酶（细胞）中酶的相对分子大小
D.固定化酶（细胞）的半衰期
2、（A）是将酶催化反应与半透膜的分离作用组合在一起而形成的反应器。
A.膜反应器
B.填充床式反应器
C.流化床式反应器
D.鼓泡式反应器
3、“结构紧凑，利于连续化生产”式那个反应器的特点（D）
A.喷射式反应器
B.鼓泡式反应器
C.搅拌罐式反应器
D.膜反应器
4、下列哪一项不是属于酶反应器应用的注意事项（C）
A.保持酶反应器的操作稳定性
B. 防止酶的变性失活
C.建立有效的目的酶跟踪监测方法
D.防止微生物污染
5、在以下的细胞破碎法中属于化学方法的是（A）
A.自溶法;B.渗透压法;C.冻融法;D.喷雾撞击法
6、下列不是固定化细胞具有的优点是（D）
A.不需要进行酶的分离
B.固定化细胞中的酶处于天然细胞的环境中，稳定性比较高。
C.保持了细胞内原有的多酶体系。
D.对不利环境的耐受性减弱，细胞不可以重复使用。
7、在酶反应器的应用过程中，应尽量保持操作的（A），避免反应条件的激烈波动
A稳定性。B实用性。C.正确性。D耐用性。
二、多选题
1.酶的固定化方法有（ABCD）
A.载体结合法;B.交联法;C.包埋法;D.新型酶固定化方法
2.突变酶是指采用基因进行（ABC），通过表达修改的基因获得的在酶学性质上符合需要的酶。
A.剪切;B.修饰;C.突变;D.基因合成
3.修饰酶的特点有（ABC）
A.热稳定性提高;B.抗原性减弱;C.半衰期延长;D.最适pH降低
三、判断题
1.酶工程是酶学、微生物学的基本原理与化学工程等有机结合而产生的交叉学科。 （√）
2.酶制备要遵循防止酶变性失活原则和建立有效的目的酶跟踪监测方法原则。 （√）
四、填空题
1.酶工程的特点是利用（酶）或（含酶细胞）作为生物催化剂完成重要的化学反应。
2.酶的来源和产生的三种方法：（提取分离法）、（化学合成法）、（生物合成法）。
五、简答题
1、有机相酶促反应的主要影响因素：
（1）含水量，（2）有机溶剂，（3）酶，（4）固定化载体，（5）pH
发布者: 李小赫
发布时间: 2022-11-21 20:40:11
1.白矾煅制的最佳温度应控制在(A)
A、180℃~260°℃
B、270°C~300℃
C、 50℃~100℃
D、120~170
2.制何首乌常用的辅料是:(2)
A、 米醋
B、 黑豆汁
C 、黄酒
D、 甘草汁
3.下列哪种物质可以用作固定化酶交联的试 剂?()
A.乙醇B.乙酸 C.戊二醛 D.乙酸乙酯
4.马钱子粉中的士的宁的含量(C) A、1.20%~2.20%
B、 1.80%~2.20%
C、 0.78%~0.82%
D、0.82%~1.20%
5.以下对药物在溶剂中溶解度无影响的因素 是(A)。
A.静置时间
B.温度
C.溶剂种类
D.溶剂组成
6.竹茹加工一般采用(D)
A、 捻捣
B、朱砂拌衣
C、 制绒
D、揉搓
7.煅后水淬的药物是（D）
A、 磁石
B、 代赭石
C、 自然铜
D 炉甘石
多选
1.以下哪些属于微生物发酵工业的发酵类型? ( ) 答案:ABCD
A.微生物菌体发酵 B.微生物酶发酵 C.微生物转化发酵 D.动、植物细胞培养
2.以下哪些是目前常用的菌种保藏方法? ( ) 答案:ABC
A.斜面低温保藏法 B.石蜡油封存法 C.液氮超低温保藏法 D.泥土保藏法
以下哪些是发酵过程的影响因素? ( ) 答案:BCD
A.发酵罐的内部结构 B.菌体浓度 C.温度 D.溶氧
填空题
1，培养基主要由（碳源），（氮源），无机盐类，生长因子，（前体物）等组成。P197
2，微生物的代谢产物按照产物的（时期），和与菌体（生长繁殖）的关系可以分为初级代谢产物和次级代谢产物。P185
判断题
（√）初级代谢产物是细胞对数期产生的代谢产物。
（×）胱氨酸可用于治疗过敏、肝炎及肝性脑病等
简答：4.简述微生物发酵制药的基本流程。
答：微生物发酵制药基本流程：
菌种选育（自然界选种、诱变育种、基因工程、细胞工程)
培养基配制(根据培养基的配制原则制备，实践中需多次试验配方)
灭菌（杀灭杂菌（胞体、孢子及芽孢）
扩大培养和接种
发酵过程(检测进程，满足营养需要；严格控制温度、pH、溶氧、转速等）
​分离纯化（菌体：过滤、沉淀；代谢产物：蒸馏、萃取、离子交换）
发布者: 牛春宇
发布时间: 2022-11-21 19:30:57
●第三章简答的正常顺序。和原来的题一样。
无血清培养基有何特点？
答：易于后期产品纯化
避免血清因素对细胞生长的抑制与毒害
减少血清中蛋白质对生物测定的干扰
减少微生物的感染
什么是原代培养？原代培养的主要步骤为啥？
答：从机体取出后立即培养的细胞为原代培养细胞，培养的第1代细胞与传10代以内的细胞称为原代细胞。
无菌条件下，从健康动物体内取出适量组织，切成小薄片。
加入适宜的胰蛋白酶或胶原蛋白酶和EDTA等进行消化作用使细胞分散
将分散细胞进行洗涤纯化后，以2×106——7×106细胞每毫升的浓度加到培养基中，37℃下进行原代培养，并适时进行传代培养。
发布者: 单晨辉
发布时间: 2022-11-20 20:51:36
第一章
单选题
1.下面不属于发酵工程技术的是（B）
A. 菌种的生产
B. 酶的固定化
C. 代谢产物的发酵
D. 菌种的选育
2.下列不属于生物技术药物分类的是（C）
A. 按用途分类
B. 按作用类型分类
C.按理化特性分类
D.按生化特性分类
3.利用酶或有机体作为催化剂实现化学转化的过程的生物技术是（A）
A.生物转化
B.糖链工程
C.酶工程
D.发酵工程
4.现代生物技术的核心和主导是（A）
A.基因工程
B.细胞工程
C.酶工程
D.发酵工程
5.生物技术制药业飞速发展的第三个十年是（C）
A.生物时代
B.技术平台时代
C.基因组时代
D.后基因组时代
6.按生化特征分类，以下生物技术药物不属于核酸类药物的是（D）
A.ATP
B.CoA
C.三氟胸苷
D.绒促性素
7.以下哪项不属于生物技术制药的生产制备特性(D)
A.制备工艺条件温和
B.分离纯化困难
C.产品易受有害物质污染
D.体内半衰期短
多选题
1.生物技术所包含的主要技术范畴有（ABCD）
A 细胞工程
B 酶工程
C 生化工程
D 发酵工程
2.下列哪些产品是用生物技术生产的（ABC）
A 青霉素
B 淀粉酶
C 乙醇
D 氯化钠
3.药理学作用特性有（ABC)
A.作用针对性强
B.毒性低
C.有种属特异性
D.体内半衰期长
判断题
1.生物技术药物的针对性弱，没有特定的作用靶分子（受体）、靶细胞和靶器官。（x）
2、生物技术药物按生化特性可分为治疗药物、预防药物、诊断药物。（x）
填空题
1.生物技术制药的特征高技术、高投入、高风险、高收益、长周期。
2.生物药物广泛应用于医学各领域、按功能用途可分为三类，分别是治疗药物、 预防药物、 诊断药物。
简答题
1.生物技术在制药中有那些应用？
生物技术应用于制药工业可大量生产廉价的防治人类重大疾病及疑难症的新型药物，具体体现在以下几个方面：
(1)基因工程制药
利用基因工程技术可生产出具有生理活性的肽类和蛋白质类药物，基因工程疫苗和抗体，还可建立更有效的药物筛选模型，改良现有发酵菌种，改进生产工艺，提供更准确的诊断技术和更有效的治疗技术等。随着基因技术的发展，应用前景会更广阔。
(2)细胞工程和酶工程制药
该技术的发展为现代制药技术提供了更强大的技术手段，使人类可控制或干预生物体初次生代谢产物和生物转化等过程，使动植物能更有效的满足人类健康方面的需求。
(3)发酵工程制药
发酵工程制药的发展主要体现在对传统工艺的改进，新药的研制和高效菌株的筛选和改造等。
发布者: 朱新录
发布时间: 2022-11-19 12:54:42
生物技术制药第六章
一、单选题
1、下列哪一项不属于固定化酶（细胞）的评价指标（C）
A.固定化酶（细胞）的活力
B.偶联率及相对活力
C.固定化酶（细胞）中酶的相对分子大小
D.固定化酶（细胞）的半衰期
2、（A）是将酶催化反应与半透膜的分离作用组合在一起而形成的反应器。
A.膜反应器
B.填充床式反应器
C.流化床式反应器
D.鼓泡式反应器
3、“结构紧凑，利于连续化生产”式那个反应器的特点（D）
A.喷射式反应器
B.鼓泡式反应器
C.搅拌罐式反应器
D.膜反应器
4、下列哪一项不是属于酶反应器应用的注意事项（C）
A.保持酶反应器的操作稳定性
B. 防止酶的变性失活
C.建立有效的目的酶跟踪监测方法
D.防止微生物污染
5、在以下的细胞破碎法中属于化学方法的是（A）
A.自溶法 B.渗透压发 C.冻融法  D.喷雾撞击法
6、下列不是固定化细胞具有的优点是（D）
A.不需要进行酶的分离
B.固定化细胞中的酶处于天然细胞的环境中，稳定性比较高。
C.保持了细胞内原有的多酶体系。
D.对不利环境的耐受性减弱，细胞不可以重复使用。
7、在酶反应器的应用过程中，应尽量保持操作的（A），避免反应条件的激烈波动
A稳定性。B实用性。C.正确性。D耐用性。
二、多选题
1.酶的固定化方法有（ABCD）
A.载体结合法  B.交联法  C.包埋法  D.新型酶固定化方法
2.突变酶是指采用基因进行（ABC），通过表达修改的基因获得的在酶学性质上符合需要的酶。
A.剪切   B.修饰   C.突变   D.基因合成
3.修饰酶的特点有（ABC）
A.热稳定性提高  B.抗原性减弱  C.半衰期延长  D.最适pH降低
三、判断题
1.酶工程是酶学、微生物学的基本原理与化学工程等有机结合而产生的交叉学科。 （√）
2.酶制备要遵循防止酶变性失活原则和建立有效的目的酶跟踪监测方法原则。 （√）
四、填空题
1.酶工程的特点是利用（酶）或（含酶细胞）作为生物催化剂完成重要的化学反应。
2.酶的来源和产生的三种方法：（提取分离法）、（化学合成法）、（生物合成法）。
五、简答题
1、有机相酶促反应的主要影响因素：
（1）含水量，（2）有机溶剂，（3）酶，（4）固定化载体，（5）pH
发布者: 杨思琪
发布时间: 2022-11-15 12:16:01
生物技术制药第八章
单选
1.(A)是微生物通过其代谢过程中产生的某一个酶或一组酶催化将一种物质(底物)转化成另一种高活性物质(产物)的一种或多种化学反应。P230
A微生物转化 B生物技术 C基因工程 D微生物反应
2.微生物转化系酶促化学反应，具有与通常有机化学反应不一样的特点，下列说法不正确的是(C)P232
A 反应条件温和
B 以具有活性中心和特殊空间结构的蛋白质酶作为催化剂
C 酶催化反应速度较慢
D对基质具有选择性和专一性
3.微生物转化有多种反应类型，其中最常见的是(D)，包括单一氧化反应、羟基化反应和氧化反应等。P231
A还原反应 B水解反应 C胺化反应 D氧化反应
4.下列属于微生物转化技术在抗癌药物生产中的应用的是(A)
A喜树碱 B阿司匹林 C洛铂 D阿法替尼
5.许多微生物都能将固醇类化合物作为(B)利用，可以将甾体母核环戊烷多氢菲和边链完全氧化为二氧化碳和水。
A氮源 B碳源 C基质 D氢供体
6.环氧化：常发生在(A)之间
A.C9，11和C14，15
B.C9，10和C14，15
C.C9，11和C13，14
D.C9，13和C14，15
7. 微生物催化的水解反应不包括(D)
A醚的开裂 B苷的水解 C水解脱胺 D脱羧反应
多选
1.萜类中应用较多的反应有（ABD）
A.酯化反应 B.闭环开环反应 C.水化反应 D.水化反应和羟化反应
2.微生物转化反应的特点（ABCD）
A以具有活性中心和特殊空间结构的蛋白质酶作为催化剂
B对作用的机制具有严格的选择性和专一性
C酶催化反应的速度极高，非一般催化剂可比
D一般在常温常压下进行反应条件温和
3甾体微生物转化反应的类型有（ABCD）
A.羟化反应 B.氧化反应 C.脱氢反应 D.甾体边链降解
填空
1.通过同源重组手段敲除或替换（ 微生物次级代谢产物）生物合成基因中特定的结构域，获得的(突变菌株)可以产生与原代谢产物不同的新化合物。 p250
2.皂苷是非糖基部分为 （三萜类 ）和 （甾体类 ）的配糖体，目前已鉴定出了1000余种皂苷，对其研究进展迅速。 p246
3.甾类化合物又称(类固醇化合)物 ，是一类生物活动中的 (重要内源性物质 ) ，在动植物及微生物中都有广泛的分布。 p234
判断
1.甾体化合物又称类固醇化合物，是一类生物活动中的重要内源性物质，在动植物中有广泛的分布，微生物中不存在。×
2.许多微生物都能够将固醇类化合物作为碳源利用，可以将甾体母核环戊烷多氢菲和边链完全氧化为二氧化碳和水。√
简答
微生物转化系酶促化学反应,具有与通常有机化学反应不一样的哪些特点。①以具有活性中心和特殊空间结构的蛋白质酶作为催化剂;②对作用的基质具有严格的选择性和专一性;③酶催化反应的速度极高，非一般催化剂可比;④一般在常温常压下进行，反应条件温和。
发布者: 陆丽丽
发布时间: 2022-11-13 16:32:14
生物制药技术第五章
单选题
1.下列关于疫苗的叙述，正确的是(C) P108
A.所有的疫苗都是安全的
B.一次使用疫苗可以预防一生
C.疫苗能迅速激发机体免疫反应
D.疫苗既可预防疾病，也可治疗疾病
2.人类历史上的第一支疫苗为（B） P109
A.卡介疫苗 B.牛痘疫苗 C.乙肝疫苗 D.炭疽疫苗
3.灭活疫苗主要通过哪种方法处理细菌或病毒研制而成（B） P122
A.萃取 B.甲醛灭活 C.冷冻灭活 D.培养
4.传统疫苗主要包括（C） P109
A.减毒活疫苗和多糖疫苗
B.灭活疫苗和重组疫苗
C.减毒活疫苗和灭活疫苗
D.减毒活疫苗和重组疫苗
5.灭活疫苗是指 ( A ) P114
A.病原微生物经培养、杀灭后只保留免疫原性而制成的疫苗
B.用毒力减弱或基本无毒的病原微生物制成的疫苗
C.利用DNA重组技术生产的疫苗
D.经处理只保留病原微生物中具有有效免疫原性的生物活性物质制成的疫苗
6.最常见的重组疫苗有（B）P126
A.甲肝疫苗 B.乙肝疫苗 C.流感疫苗 D.狂犬病疫苗
7.亚单位疫苗的特点是（D） P116
A.纯度不高 B.免疫效果差 C.不良反应高 D.免疫原性低，需与佐剂合用
多选题
1.下列属于新型疫苗的是（ ）CD p118
A、灭活疫苗 B、亚单位疫苗
C、治疗性疫苗 D、DNA疫苗
2.生物技术疫苗与传统疫苗相比，其优点有（ ）ABCD. p134
A、减毒更彻底B、遗传特性更稳定C、安全性强D、不易发生毒力回复
3.哪些疫苗接种途径为皮下注射（ ） ABCD p136
A、麻疹疫苗
B、乙脑减毒活疫苗
C、乙脑灭活疫苗
D、A+G流脑疫苗
E、卡介苗
填空题
1.按照制备技术或特点可将疫苗大体分为六类,分别为:（减毒活疫苗）（灭活疫苗）（亚单位疫苗）联合疫苗、核酸疫苗和治疗性疫苗。p113
2.疫苗的组成是具有（免疫保护性的抗原）如蛋白质、多肽、多糖或核酸等，与（免疫佐剂）混合制备而成。p112
判断题
1.流感疫苗于流感流行季节前或期间进行预防接种，儿童和成人均于上臂三角肌肌内注射 ，也可静脉注射。（×）
2.亚单位疫苗是除去病原体中无免疫保护作用的有害成分，保留其有效的免疫原成分制成的疫苗。（✓）p116
简答题
1.简述疫苗的概念及其作用原理。P108 p112
答：（1)是将病原微生物（如细菌、立克次氏体、病毒等） 及其代谢产物，经过人工减毒、灭活或利用基因工程等方法制成的用于预防传染病的自动免疫制剂。
（2） 当机体通过注射或口服等途径接种疫苗后，疫苗中的抗原分子就会发生免疫原性作用，刺激机体免疫系统产生高效价特异性的免疫保护物质，如特异性抗体、免疫细胞及细胞因子等，当机体再次接触到相同的抗原时候，机体的免疫系统便会依循免疫记忆，迅速制造出更多的保护物质来阻断病原菌的入侵，从而使机体获得针对病原体特异性的免疫力，使其免受侵害而得到保护。
发布者: 梁玉璇
发布时间: 2022-11-13 13:12:34
第4章
单选
1.下列哪个不是第三代基因工程抗体的优点？D
A.均一性强 B.可工业化生产 C.人源化
D.效价高
2.哪个不属于噬菌体抗体的筛选方法？A
A.融合蛋白筛选
B.利用固相纯化抗原抗体进行筛选 C.全细胞筛选
D.用切片组织进行筛选
3.脾细胞和骨髓瘤细胞化学法用( )试剂融合？B
A.AS B.PEG C.EC D.HPC
4.初次免疫应答时获得的杂交瘤主要分泌( )抗体？A
A.IgM B.IgG C.IgA D.IgE
5.再次，免疫应答时获得的杂交瘤细胞主要分泌( )抗体? C
A.IgD B.IgE C.IgG D.IgM
6.完整的抗体不包括下列哪个？ D
A.鼠源性单抗 B.嵌合抗体
C.人源化抗体 D.抗体偶联物
7.体外免疫的优点？C
A抗体含量浓度高 B.不需要重复无菌操作
C.所需抗原量少 C.可持续抽取
多选
1.阳性克隆的筛选方法有? ABCD
A.ELISA B.KIA C.FACS D.IFA
2.完整的抗体包括下列哪个？ ABC
A.人体抗 B.鼠源性单抗
C.人源化抗体 D.抗体融合蛋白
3.下列哪个属于单抗的鉴定？ ABCD
A.抗体特异性的鉴定
B.单抗的类与亚类的鉴定
C.单抗中和活性的鉴定
D.单抗亲和力的鉴定
填空
1.动物免疫方法分为（体外、体内、脾内免疫法）
2.抗体分子具有一个共同结构，即由（4条多肽链）通过（二硫键）连接而成。
判断：
1、抗体工程制药是应用细胞工程或基因工程等生物技术制备抗体药物的一门学科（✓）
2、抗体由4条多肽链通过二硫键连接而成，为“Y”字形结构，有两个主要功能，一是识别并结合抗原，二是结合后的免疫应答。（✓）
简答
制备单抗时为什么要选用B淋巴细胞与骨髓瘤细胞融合形成杂交瘤细胞？
答：骨髓瘤细胞在体外培养能大量无限增殖，但不能分泌特异性抗体；而抗原免疫的B细胞能产生特异性抗体，但在体外不能无限增殖。将免疫B细胞与骨髓瘤细胞融合后形成的杂交瘤细胞，继承了两个亲代细胞的特性，既具有骨髓瘤细胞能无限增殖的特性，又具有免疫B细胞合成和分泌特异性抗体的能力。
发布者: 严紫婷
发布时间: 2022-11-11 21:58:44
制药技术制药第九章
单选题
1.人血清蛋白是目前研究最多的一种蛋白质化学修饰剂，其修饰方法主要有戊二醛法、活性脂法、( B )等。P280
A.异氰酸法 B.碳二亚胺法 C.高碘酸钠氧化法 D.溴化氰活化法
2.SOD在体内的半衰期很短，仅6~10分钟，因此不宜维持长时间的治疗作用，通过化学修饰，可大大延长其半衰期，其中PEG-SOD的半衰期为( D )。P286
A.8h B.16h C.24h D.40h
3.高pH时，溴化氢可以与PEG的( A )反应转化成氰酸酯何亚氨基碳酸酯，从而实现PEG的活化，然后再与蛋白质偶联，实现化学修饰。P272
A.羟基 B.氨基 C.巯基 D.羧基
4.糖类用于修饰蛋白质之前，需要经过活化目前常用的活化剂有( B )、溴化氰、氰脲酰氯和碳二亚胺等。P278
A.硝酸铅 B.高碘酸钾 C.硫酸铜 D.氢氧化钠
5.蛋白质药物的化学修饰的说法正确的是( A )。
A.主要目的是为了延长药物分子在体内的半衰期
B.修饰剂阻碍蛋白质药物的稳定性
C.主要修饰剂为多肽类化合物
D.主要是对蛋白质侧链进行改变
6.一般 PEG的Mr越大，修饰蛋白质的活性损失( A )。
A.越大 B.越小 C.没有影响 D.有时候大，有时候小
7.以下哪一项不能体现蛋白质药物化学修饰赋予蛋白质药物的优良性能( D )。
A.循环半衰期延长
B.免疫原性降低或消失，毒副作用减少。
C.物理、化学和生物稳定性增强
D.制药成本降低
多选题
1.定点修饰可选定的修饰靶点为（ABCD）P274
A.氨基酸修修饰 B.羧基修饰 C.巯基修饰 D.非天然氨基酸修饰
2.可作为修饰剂的糖（ABCD）P277
A. 右旋糖酐类 B.甘露聚糖 C.肝素类 D.壳聚糖
填空题
1.蛋白质的化学修饰，主要包括（侧链基团）的改变和（主链结构）改变两个方面。P266
2.PEG类修饰剂可以经（氰脲酰氯）、（二环己基碳二亚胺（DCC））等活化剂活化后，再与蛋白质共价结合。P268
判断题
1.蛋白质是一类重要的生物大分子，其生物活性取决于其特定的三级结构和空间结构。（×）p266
2.蛋白质的PEG修饰，是将活化的PEG通过化学方法以共价键偶联到蛋白质上的技术。（✓）
简答题
1.简述PEG定点修饰蛋白质要考虑的因素。P274
答:PEG定点修饰蛋白质主要从三个方面着手：①蛋白质方面，通过蛋白质的定点突变、蛋白质可逆性位点定向保护、非天然氨基酸的引入等，以引入或留有单一的供修饰的基团；②PEG的活化形式，即通过选择PEG的活化形式来实现与特定基团的反应，如修饰氨基的PEG-醛和修饰巯基的PEG-乙烯基砜等；③反应条件的控制，即通过控制pH、温度、金属离子或酶的催化等实现定向修饰反应。
发布者: 黎冰冰
发布时间: 2022-11-05 13:16:03
生物技术制药第二章
单选题
1.以大肠杆菌为目的基因的表达体系，下列正确的是（C）
A、表达产物为糖基化蛋白质 B、表达产物存在的部位是在菌体内
C、容易培养，产物提纯简单 D、表达产物为天然产物
2.人类第一个基因工程药物是（A）P8
A、人胰岛素 B、重组链激酶
C、促红细胞生成素 D、乙型肝炎疫苗
3.cDNA法获得目的基因的优点是（B）P12
A、成功率高 B、不含内含子 C、操作简便 D、表达产物可以分泌
4.基因表达最常用的宿主菌是（A）
A、大肠杆菌 B、枯草芽孢杆菌 C、链霉菌 D、酵母
5.基因工程的单元操作顺序是（A）p11-18
A、酶切，连接，转化，筛选，验证
B、酶切，转化，连接，筛选，验证
C、连接，转化，筛选，验证，酶切
D、验证，酶切，连接，筛选，转化
6.基因工程原核表达体系拥有比其他表达体系更多的优点，以下不属于原核表达体系的优点的是（B）p19
A、操作简便、繁殖快、周期短
B、下游纯化工艺复杂，生产效率低
C、宿主菌遗传背景清楚，商品化菌种齐全，方便购买
D、大规模生产成本低，产量较高
7.哺乳动物细胞表达系统中，宿主细胞最常用的一种为（D）p23
A、COS细胞 B、鼠骨髓瘤细胞株
C、Vero细胞 D、CHO细胞
多选题
1.产物的分离技术多种多样，其中属于沉淀分离的是（AC）p25
A、等电点沉淀法 B、盐溶法 C、盐析法 D、离心沉降法
2.重组DNA导入细菌的方法包括（ABC）p16
A、化学转化法 B、电转化法 C、感染法 D、基因枪法
3.基因工程药物质量控制项目主要包括（ABCD）p32
A、蛋白质含量测定 B、蛋白质纯度检查
C、蛋白质理化性质的鉴定 D、生物活性测定
判断题
基因重组蛋白质的分离提纯过程中，温度应控制在低温条件中，整个分离过程应缓慢进行。（×）p24
填空题
1.（构建突变体）和（构建融合蛋白）是改善和提高天然活性蛋白质药物性质的手段。p30-31
2.目的基因的获取方法包括（化学合成法）、（PCR法）、（cDNA文库法）和（基因组DNA文库法）p11
简答题
简述基因工程制药的基本流程?p9
1、目的基因的获取 2、表达载体的选择 3、目的基因与载体的链接 4、重组DNA转入受体细胞 5、重组子的筛选与鉴定 6、工程菌株发酵表达重组蛋白 7、重组蛋白产品的纯化 8、重组蛋白制剂的生产
发布者: 王晴
发布时间: 2022-11-04 22:18:05
生物技术制药第三章
单选题
1.动物细胞培养的常用条件（ C ）
A.25℃，8%二氧化碳，92%氧气
B.25℃，5%二氧化碳，95%氧气
C.37℃，5%二氧化碳，95%氧气
D.37℃，8%二氧化碳，92%氧气
2、生产用动物细胞培养技术的要求有连续生产能力，产物产量高，培养条件易于控制的（ A ）
A.细胞系 B.原代细胞 C.继代细胞 D.无限传代细胞
3、 以下不是动物细胞工程制药技术的是（ D ）
A.细胞融合技术 B.基因转移技术 C.细胞大规模培养技术 D.细胞种质保存技术
4、动物细胞培养的最适pH为（ D ）
A.6.5-6.8 B.7.0-7.2 C.6.8-7.0 D.7.2-7.4
5.动物细胞工程制药是指利用(A)为宿主或反应器，进行疫苗，多肽和蛋白质等生物制品的生产。
A.动物细胞 B.植物细胞 C.细菌 D，.微生物
6、非贴壁依赖性细胞 这类细胞一般是来源于血液、（B）的细胞和杂交瘤细胞。A.上皮组织 B.淋巴组织 C.肌肉组织 D.结缔组织
7、若向培养液加入（B），可使冰点降低。在缓慢的冻结条件下，能使细胞内的水分在冻结前透出细胞。
A. 缓冲剂 B. 保护剂 C. 降温剂 D. 润滑剂
多选
1、动物细胞工程制药主要用于生产适合真核细胞表达的各类药物，其中包括（ABCD）
A.疫苗 B.干扰素 C.激素 D.生长因子
2.生产用的动物细胞需要满足以下培养技术要求（ABD）
A.具有连续生长能力B.目的产物产量很高C.必须是贴壁细胞D.培养条件易选择控制3、动物细胞培养过程中，需要考虑的环境要求主要涉及到（ABCD）
A.培养温度B.气体环境C.营养物质D.渗透压
填空
1.动物细胞培养基是维持动物组织及细胞在体外生存、生长的基本营养物质，从发展历史看，可分为(天然培养基)、(合成培养基)、无血清培养基。
2.动物细胞实验室的培养方法可分为(悬浮培养)、(贴壁培养)、和贴壁—悬浮培养。
判断
1.动物细胞培养与微生物细胞培养基本原理相同，营养要求和环境条件相进（ × ）
2.动物细胞体外培养的基本过程：从有机体取得材料——切碎或酶解——原代培养——传代培养。（√ ）
简答
1.无血清培养基有何特点？
答：易于后期产品纯化避免血清因素对细胞生长的抑制与毒害减少血清中蛋白质对生物测定的干扰减少微生物的感染什么是原代培养？原代培养的主要步骤为啥？答：从机体取出后立即培养的细胞为原代培养细胞，培养的第1代细胞与传10代以内的细胞称为原代细胞。无菌条件下，从健康动物体内取出适量组织，切成小薄片。加入适宜的胰蛋白酶或胶原蛋白酶和EDTA等进行消化作用使细胞分散将分散细胞进行洗涤纯化后，以2×106——7×106细胞每毫升的浓度加到培养基中，37℃下进行原代培养，并适时进行传代培养。
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